Microbial-Based Biopesticides by Travis R. Glare & Maria E. Moran-Diez

Author:Travis R. Glare & Maria E. Moran-Diez
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

5.Cryopreserved cells should have recovered (i.e., normal cell growth rate and viability) by 2–3 passages after thaw. If not, then discard the cells and thaw out another vial from the cell bank. If there is a low success rate of recovering cells from a particular cryopreserved batch, then the freezing procedure may not have been carried out properly. In this case, repeat the cryopreservation procedure with a new batch of cells.

6.Insect cells will obviously exhibit different growth characteristics depending on the medium used. One of the most important initial tasks in insect cell culture is to establish a cell growth curve (cell density and viability over time) for a particular medium, from which the cell doubling time, mid-exponential growth phase, and PCD can be determined. This information is used to set the cell density and temporal parameters for passaging events.

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